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Analysis of institutional authors

Blanco-Romero, EstherAuthorGarrido-Sanz, DanielAuthorDurán, DavidAuthorRedondo-Nieto, MiguelAuthorRivilla, RafaelAuthorMartin, MartaCorresponding Author
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Role of extracellular matrix components in biofilm formation and adaptation of Pseudomonas ogarae F113 to the rhizosphere environment

Publicated to:Frontiers in Microbiology. 15 1341728- - 2024-01-25 15(), DOI: 10.3389/fmicb.2024.1341728

Authors: Blanco-Romero, Esther; Garrido-Sanz, Daniel; Duran, David; Rybtke, Morten; Tolker-Nielsen, Tim; Redondo-Nieto, Miguel; Rivilla, Rafael; Martin, Marta

Affiliations

Univ Autonoma Madrid, Fac Ciencias, Dept Biol, Madrid, Spain - Author
Univ Copenhagen, Costerton Biofilm Ctr, Dept Immunol & Microbiol, Copenhagen, Denmark - Author
Univ Lausanne, Dept Fundamental Microbiol, Lausanne, Switzerland - Author

Abstract

Regulating the transition of bacteria from motile to sessile lifestyles is crucial for their ability to compete effectively in the rhizosphere environment. Pseudomonas are known to rely on extracellular matrix (ECM) components for microcolony and biofilm formation, allowing them to adapt to a sessile lifestyle. Pseudomonas ogarae F113 possesses eight gene clusters responsible for the production of ECM components. These gene clusters are tightly regulated by AmrZ, a major transcriptional regulator that influences the cellular levels of c-di-GMP. The AmrZ-mediated transcriptional regulation of ECM components is primarily mediated by the signaling molecule c-di-GMP and the flagella master regulator FleQ. To investigate the functional role of these ECM components in P. ogarae F113, we performed phenotypic analyses using mutants in genes encoding these ECM components. These analyses included assessments of colony morphology, dye-staining, static attachment to abiotic surfaces, dynamic biofilm formation on abiotic surfaces, swimming motility, and competitive colonization assays of the rhizosphere. Our results revealed that alginate and PNAG polysaccharides, along with PsmE and the fimbrial low molecular weight protein/tight adherence (Flp/Tad) pilus, are the major ECM components contributing to biofilm formation. Additionally, we found that the majority of these components and MapA are needed for a competitive colonization of the rhizosphere in P. ogarae F113.

Keywords
AeruginosaAlginate productionBacteriaBiofilmColonizationDynamic biofilm formationEscherichia-coliExopolysaccharideExtracellular matrix componentsFlow cellFluorescensGenesMotilityO-acetylationPseudomonas ogarae f113PutidaRhizosphereSurface attachment

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Frontiers in Microbiology due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Microbiology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-05-07:

  • WoS: 2
  • Scopus: 5
  • Europe PMC: 2
  • OpenCitations: 3
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-07:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 20.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 19 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 8.33.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/716451
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Denmark; Switzerland.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (BLANCO ROMERO, ESTHER) and Last Author (MARTIN BASANTA, MARTA).

the author responsible for correspondence tasks has been MARTIN BASANTA, MARTA.