{rfName}
DN

Indexed in

License and use

Icono OpenAccess

Citations

26

Altmetrics

Analysis of institutional authors

Redrejo-Rodríguez MCorresponding Author

Share

Publications
>
Review

DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions

Publicated to:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 24 (11): 9331- - 2023-06-01 24(11), DOI: 10.3390/ijms24119331

Authors: Ordonez, Carlos D; Redrejo-Rodriguez, Modesto

Affiliations

CIC bioGUNE - Author
CIC BioGUNE, Bizkaia Sci & Technol Pk,Bldg 800, Derio 48160, Spain - Author
CSIC UAM, Inst Invest Biomed Alberto Sols, Madrid 28029, Spain - Author
CSIC-UAM - Instituto de Investigaciones Biomédicas Alberto Sols (IIBM) - Author
Univ Autonoma Madrid, CSIC UAM, Dept Biochem, Madrid 28029, Spain - Author
See more

Abstract

In the same way that specialized DNA polymerases (DNAPs) replicate cellular and viral genomes, only a handful of dedicated proteins from various natural origins as well as engineered versions are appropriate for competent exponential amplification of whole genomes and metagenomes (WGA). Different applications have led to the development of diverse protocols, based on various DNAPs. Isothermal WGA is currently widely used due to the high performance of Φ29 DNA polymerase, but PCR-based methods are also available and can provide competent amplification of certain samples. Replication fidelity and processivity must be considered when selecting a suitable enzyme for WGA. However, other properties, such as thermostability, capacity to couple replication, and double helix unwinding, or the ability to maintain DNA replication opposite to damaged bases, are also very relevant for some applications. In this review, we provide an overview of the different properties of DNAPs widely used in WGA and discuss their limitations and future research directions.

Keywords

abasic sitesbinding domainbst dna polymerasedna polymeraseenzymatic amplificationescherichia-colifidelitygenetic informationin-vitromultiple displacement amplificationpcrphi 29 dna polymeraseprocessivitypyrococcus-furiosustranslesion synthesiswhole genome amplificationy-familyBst dna polymeraseDnaDna polymeraseDna-directed dna polymeraseFidelityGenome, viralHairpin-helix motifsMultiple displacement amplificationNucleic acid amplification techniquesPcrPolymerase chain reactionProcessivityTranslesion synthesisWhole genome amplificationΦ29 dna polymerase

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 66/313, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 5.8, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-10, the following number of citations:

  • WoS: 8
  • Scopus: 10
  • Europe PMC: 8
  • OpenCitations: 10

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-10:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 37.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 36 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 11.15.
  • The number of mentions on the social network X (formerly Twitter): 12 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/10486/708597

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (REDREJO RODRIGUEZ, MODESTO).

the author responsible for correspondence tasks has been REDREJO RODRIGUEZ, MODESTO.