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Analysis of institutional authors

Martín González, NataliaAuthorHernando-Perez MAuthorPérez-Illana MAuthorDe Pablo PjAuthor

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August 20, 2019
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Article

Adenovirus major core protein condenses DNA in clusters and bundles, modulating genome release and capsid internal pressure

Publicated to: NUCLEIC ACIDS RESEARCH. 47 (17): 9231-9242 - 2019-09-26 47(17), DOI: 10.1093/nar/gkz687

Authors:

Martín-González, N; Hernando-Pérez, M; Condezo, GN; Pérez-Illana, M; Siber, A; Reguera, D; Ostapchuk, P; Hearing, P; San Martín, C; de Pablo, PJ
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Affiliations

CSIC, CNB, Dept Macromol Struct, E-28049 Madrid, Spain - Author
Departament de Física de la Matèria Condensada, Facultat de Física, Universitat de Barcelona, Martí i Franqués 1, 08028 Barcelona, Spain - Author
Department of Condensed Matter Physics, Universidad Autónoma de Madrid, Madrid 28049, Spain - Author
Department of Macromolecular Structures, Centro Nacional de Biotecnología (CNB-CSIC), Madrid 28049, Spain - Author
Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794-5222, USA - Author
Inst Phys, Zagreb, Croatia - Author
Institute of Physics, Zagreb, Croatia - Author
Instituto de Física de la Materia Condensada (IFIMAC), Universidad Autónoma de Madrid, Madrid 28049, Spain - Author
SUNY Stony Brook, Sch Med, Dept Mol Genet & Microbiol, Stony Brook, NY 11794 USA - Author
UBICS, Barcelona 08028, Spain - Author
Univ Autonoma Madrid, Dept Condensed Matter Phys, E-28049 Madrid, Spain - Author
Univ Autonoma Madrid, Inst Fis Mat Condensada IFIMAC, E-28049 Madrid, Spain - Author
Univ Barcelona, Fac Fis, Dept Fis Mat Condensada, Marti & Franques 1, E-08028 Barcelona, Spain - Author
Universitat de Barcelona Institute of Complex Systems (UBICS), 08028 Barcelona, Spain - Author
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Abstract

Some viruses package dsDNA together with large amounts of positively charged proteins, thought to help condense the genome inside the capsid with no evidence. Further, this role is not clear because these viruses have typically lower packing fractions than viruses encapsidating naked dsDNA. In addition, it has recently been shown that the major adenovirus condensing protein (polypeptide VII) is dispensable for genome encapsidation. Here, we study the morphology and mechanics of adenovirus particles with (Ad5-wt) and without (Ad5-VII-) protein VII. Ad5-VII- particles are stiffer than Ad5-wt, but DNA-counterions revert this difference, indicating that VII screens repulsive DNA-DNA interactions. Consequently, its absence results in increased internal pressure. The core is slightly more ordered in the absence of VII and diffuses faster out of Ad5-VII- than Ad5-wt fractured particles. In Ad5-wt unpacked cores, dsDNA associates in bundles interspersed with VII-DNA clusters. These results indicate that protein VII condenses the adenovirus genome by combining direct clustering and promotion of bridging by other core proteins. This condensation modulates the virion internal pressure and DNA release from disrupted particles, which could be crucial to keep the genome protected inside the semi-disrupted capsid while traveling to the nuclear pore.© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
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Keywords

AdenoviridaeArchitectureAtomic-force microscopyCapsid proteinsCryo-em structureDna, viralEntryGenome, viralHumansMaturationMechanical-propertiesParticlesRevealsTransitionViral core proteinsViral proteinsVirionVirusVirus assembly

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal NUCLEIC ACIDS RESEARCH due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position 15/297, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.02. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.28 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-06, the following number of citations:

  • WoS: 31
  • Scopus: 35
  • Europe PMC: 15
  • Google Scholar: 42
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-06:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 40.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 40 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.
  • The number of mentions on the social network X (formerly Twitter): 5 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/716038
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Croatia; United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MARTIN GONZALEZ, NATALIA) and Last Author (DE PABLO GOMEZ, PEDRO JOSE).

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Awards linked to the item

Spanish Ministry of Economy and Competitivity [FIS201789549-R; Maria de Maeztu Program for Units of Excellence in R&D MDM-2014-0377; and FIS2017-90701-REDT] and Human Frontiers Science Program [HFSPO RGP0012/2018] to P.J.P. Spanish State Research Agency and European Regional Development Fund [BFU2016-74868-P] and Spanish Ministry of Economy, Industry and Competitivity [BIO2015-68990-REDT, the Spanish Adenovirus Network, AdenoNet] to C.S.M. MINECO/FEDER, UE [FIS2015-67837-P] to D.R. National Institutes of Health [CA122677 and AI102577] to P.H. M.H.-P. is a recipient of a Juan de la Cierva postdoctoral contract funded by the Spanish Ministry of Economy and Competitivity. M.P.-I. holds a predoctoral fellowship funded by La Caixa Foundation. Funding for open access charge: Secretar ' ia de Estado de Investigacion, Desarrollo e Innovacion [FIS2017-89549-R].
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