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Analysis of institutional authors

Núñez, EAuthorGomez-Lopez, SAuthorDe Juan-Sanz, JCorresponding AuthorAragon, CAuthor

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November 1, 2021
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The presynaptic glycine transporter GlyT2 is regulated by the Hedgehog pathway in vitro and in vivo

Publicated to: Commun Biol. 4 (1): 1197- - 2021-12-01 4(1), DOI: 10.1038/s42003-021-02718-6

Authors: De la Rocha-Munoz, Andres; Nunez, Enrique; Vishwanath, Anjali Amrapali; Gomez-Lopez, Sergio; Dhanasobhon, Dhanasak; Rebola, Nelson; Lopez-Corcuera, Beatriz; De Juan-Sanz, Jaime; Aragon, Carmen;

Affiliations

Hosp Univ La Paz, Idipaz, Madrid, Spain - Author
Sorbonne Univ, Inst Cerveau, Hop Pitie Salpetriere, AP HP,Paris Brain Inst,ICM,INSERM,CNRS, Paris, France - Author
Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, CSIC, Madrid 28049, Spain - Author
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Abstract

The identity of a glycinergic synapse is maintained presynaptically by the activity of a surface glycine transporter, GlyT2, which recaptures glycine back to presynaptic terminals to preserve vesicular glycine content. GlyT2 loss-of-function mutations cause Hyperekplexia, a rare neurological disease in which loss of glycinergic neurotransmission causes generalized stiffness and strong motor alterations. However, the molecular underpinnings controlling GlyT2 activity remain poorly understood. In this work, we identify the Hedgehog pathway as a robust controller of GlyT2 expression and transport activity. Modulating the activation state of the Hedgehog pathway in vitro in rodent primary spinal cord neurons or in vivo in zebrafish embryos induced a selective control in GlyT2 expression, regulating GlyT2 transport activity. Our results indicate that activation of Hedgehog reduces GlyT2 expression by increasing its ubiquitination and degradation. This work describes a new molecular link between the Hedgehog signaling pathway and presynaptic glycine availability.By modulating the activation state of the Hedgehog pathway, de la Rocha-Munoz et al demonstrate that Hedgehog signaling controls the expression and transport activity of the neuronal glycine transporter GlyT2. This work begins to reveal a potential link between the Hedgehog signaling pathway and presynaptic glycine availability.

Keywords

Developmental expressionDopamine transporterEndocytosisGlutamate transporterKinase-cLocalizationNeuronsProteinSonic-hedgehogUbiquitination

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Commun Biol due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position 15/94, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-06:

  • WoS: 2
  • Scopus: 3
  • Europe PMC: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-06:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 8.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 7 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 4 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/704536

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: France.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (De la Rocha-Munoz, A) and Last Author (ARAGON RUEDA, CARMEN).

the author responsible for correspondence tasks has been DE JUAN SANZ, JAIME.

Awards linked to the item

This work was supported by grants from the Spanish 'Ministerio de Economia y Competitividad', grant number SAF2017-84235-R (AEI/FEDER, EU) to B.L.-C. and from Fundacion Ramon Areces to B.L.-C. Additionally, Institutional grants from the Fundacion Ramon Areces and Banco de Santander are given to the CBMSO. J.d.J.-S receives support from the Diane Barriere Chair on Synaptic Bioenergetics (ICM Paris Brain Institute). J.d.J.-S is supported by the 2019 ATIP-Avenir program (Inserm and CRNS, France) and an ERC Starting Grant (ERC-StG-852873, EU). We thank Dr. Fernando Martin-Belmonte (Centro de Biologia Molecular Severo Ochoa [CBMSO]) for generously providing the zebrafish embryos and experimental advice.