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The authors wish to thank Professor Gerard Tramu (Universite de Bordeaux I, Bordeaux, France) for the gift of primary antibodies and the Language Service of the Universidad de Salamanca (Spain) for supervising the English text. This work has been supported by "Programa XI: Financiacion de Unidades de Excelencia de la Universidad de Salamanca" (Spain).

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Sanchez, PabloAuthor
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Neuroanatomical distribution of the enkephalinergic and tachykininergic systems in the alpaca brainstem: an immunohistochemical study

Publicated to:FOLIA HISTOCHEMICA ET CYTOBIOLOGICA. 59 (3): 145-156 - 2021-01-01 59(3), DOI: 10.5603/FHC.a2021.0016

Authors: Sanchez, Pablo; Lisardo Sanchez, Manuel; Mangas, Arturo; de Souza, Eliana; Angel Aguilar, Luis; Covenas, Rafael

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Grp GIR USAL BMD Bases Moleculares Desarrollo, Salamanca, Spain - Author
San Ignacio de Loyola Univ USIL, Fac Hlth Sci, Lima, Peru - Author
Univ Salamanca, Inst Neurosci Castilla & Leon INCYL, Lab Neuroanat Peptiderg Syst Lab 14, C Pintor Fernando Gallego 1, Salamanca 37007, Spain - Author

Abstract

Introduction. A recent study has shown a close neuroanatomical relationship between the enkephalinergic (methionine-enkephalin) and tachykininergic (substance P) systems in the alpaca diencephalon. In this study, our aim is to show this relationship in the alpaca brainstem. Material and methods. Using an immunohistochemical technique, the distribution of immunoreactive (Ir) fibers and cell bodies containing substance P (SP) or methionine-enkephalin (MET) has been studied in the alpaca brainstem. Five adult males were used; brain tissue was fixed and processed by standard methods. Results. SP- and MET-Ir fibers showed a widespread and similar distribution in the mesencephalon, pons and medulla oblongata. The co-localization of fibers containing SP or MET was found in most of the nuclei/tracts of the alpaca brainstem. This close neuroanatomical relationship suggests multiple physiological interactions between both neuropeptides. The distribution of the cell bodies containing SP was very restricted (cell bodies were only observed in a few nuclei located in the mesencephalon and medulla oblongata), whereas MET-Ir perikarya showed a moderately widespread distribution in the mesencephalon, pons and medulla oblongata. Conclusions. This study increases the knowledge on the neuroanatomical distribution/relationship of the tachykininergic (SP) and enkephalinergic (MET) systems in the alpaca central nervous system.

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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Peru.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (SANCHEZ MARTIN, PABLO) .