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This work was financed by Grants PID2020-116192RB-I00 funded by MCIN/AEI/10.13039/501100011033; TED2021-129937B-I00, CNS2022-135495, and CNS2023-145169 funded by MCIN/AEI/10.13039/501100011033; and the European Union NextGenerationEU/PRTR. The authors would like to acknowledge Dr. Malou Henriksen-Lacey and Prof. Luis Liz-Marzan, from CIC BiomaGUNE (San Sebastian, Spain), for supplying the cell lines. P.C. thanks the regional government of Comunidad de Madrid for the Programa Investigo (Plan de Recuperacion, Transformacion y Resiliencia) which was developed thanks to SEPE, Ministerio de Trabajo y Economia Social and the European Union through NextGenerationEU.

Analysis of institutional authors

Camarero, PabloAuthorQuintanilla, MartaAuthorGarcia-Cabanes, AngelAuthorHaro-Gonzalez, PatriciaAuthorCarrascosa, MercedesCorresponding Author

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November 4, 2024
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Manipulation of Cancer Cell Spheroids by Photovoltaic Tweezers: Determination of Their Charge State

Publicated to:ADVANCED PHOTONICS RESEARCH. - 2024-10-20 (), DOI: 10.1002/adpr.202400124

Authors: Rincon, Esther; Camarero, Pablo; Quintanilla, Marta; Mendez, Angel; Garcia-Cabanes, Angel; Haro-Gonzalez, Patricia; Carrascosa, Mercedes

Affiliations

Univ Autonoma Madrid, Dept Fis Mat, Madrid 28049, Spain - Author
Univ Autonoma Madrid, Inst Nicolas Cabrera, Madrid 28049, Spain - Author
Univ Politecn Madrid, Dept Mecan Fluidos & Prop Aerosp, E-28040 Madrid, Spain - Author

Abstract

Photovoltaic optoelectronic tweezers (PVOT), based on the bulk photovoltaic effect presented by certain ferroelectric crystals, have emerged as a versatile tool to manipulate and/or trap micro- and nano-objects. Very recently, an ingenious method has enabled to extend PVOT manipulation to aqueous solution droplets opening the door to applications in optofluidics and biotechnology. The photovoltaic crystal is placed on the bottom of a cuvette filled with paraffin oil while the droplet is hanging from the air-oil interphase where it is manipulated. Herein, this method is used to investigate the feasibility of manipulating hybrid droplets as carriers containing a cancer multicellular spheroid inside cell culture medium. Droplets of two cancer cell lines, MCF-7 and U-87 MG, have been successfully manipulated by light-induced electrical fields generated in the photovoltaic platform. From the droplet dynamics, which includes attractive and repulsive behaviors from the illuminated crystal region, the charge state of the cell culture medium and the spheroid are investigated. The results show that they are positively and negatively charged, respectively. Moreover, analyzing the migration kinetics of water, cell culture medium, and hybrid droplets, to the light of a proposed theoretical model, the charge density of both cell line spheroids has been estimated. Droplets containing spheroids of two cancer cell lines, MCF-7 and U-87-MG, have been successfully manipulated by light-induced electrical fields generated by photovoltaic tweezers. From the droplet dynamics, the charge state of the cell culture medium and the spheroids are obtained resulting in positively and negatively charged, respectively. Moreover, the charge density of the spheroids of both cell lines has been estimated.image (c) 2024 WILEY-VCH GmbH

Keywords

Droplet optofluidicsElectrophoresiFerroelectric platformsMulticellular spheroidsOptoelectronic manipulationsPhotovoltaic tweezerSingle cellsWater microdroplets

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal ADVANCED PHOTONICS RESEARCH due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 176/439, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Materials Science, Multidisciplinary.

Impact and social visibility

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/715863

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Rincon, Esther) and Last Author (CARRASCOSA RICO, MERCEDES).

the author responsible for correspondence tasks has been CARRASCOSA RICO, MERCEDES.